Semaphorin 3B (SEMA3B) Rabbit Polyclonal Antibody

CAT#: TA309848

Rabbit Polyclonal SEMA3B Antibody

Product Images

Immunocytochemistry/Immunofluorescence: Semaphorin 3B Antibody TA309848 - SEMA3B antibody was tested in Neuro-2a cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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  Simple Western: Semaphorin 3B Antibody TA309848 - Simple Western lane view shows a specific band for SEMA3B in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Western Blot: Semaphorin 3B Antibody TA309848 - Western blot analysis of SEMA3B in A. Hela WCE B. Ntera2 C. A431 D. HepG2 E. MCF7 F. NIH/3T3 G. PC12 H. Cos7

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  Immunocytochemistry/Immunofluorescence: Semaphorin 3B Antibody TA309848 - SEMA3B antibody (1:50) was tested in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.

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  Immunohistochemistry: Semaphorin 3B Antibody TA309848 - IHC analysis of SEMA3B in mouse kidney using DAB with hematoxylin counterstain.

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  Immunohistochemistry-Paraffin: Semaphorin 3B Antibody TA309848 - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse kidney using 1:200 dilution of rabbit anti-Semaphorin 3B antibody. This antibody generated a weak to moderate cytoplasmic staining in the cells of various renal tubules and glomeruli.

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Specifications

Product Data

• Applications: ICC/IF, IHC, Simple Western, WB
• Recommended Dilution: Western Blot: 1:500-1:2000, Immunohistochemistry: 1:100 - 1:200, Immunohistochemistry-Paraffin: 1:100 - 1:200, Immunocytochemistry/ Immunofluorescence: 1:40, Simple Western: 1:25
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal portion of the human SEMA 3B protein sequence (between residues 100-200).
• Formulation: Tris-glycine, 150mM NaCl and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: semaphorin 3B
• Database Link:
  NP_004627
  Entrez Gene 20347 MouseEntrez Gene 363142 RatEntrez Gene 7869 Human
  UniProt ID Q13214
• Synonyms: LUCA-1; SemA; SEMA5; SEMAA; semaV

Reference Data

• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Axon guidance