NeuN (RBFOX3) Rabbit Polyclonal Antibody

CAT#: TA309832

Rabbit Polyclonal RBFOX3/NeuN Antibody

Product Images

Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody TA309832 - NeuN antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

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  Flow Cytometry: RBFOX3/NeuN Antibody TA309832 - An intracellular stain was performed on Hek293 cells with TA309832AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

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  Flow Cytometry: RBFOX3/NeuN Antibody TA309832 - An intracellular stain was performed on U2OS cells with RBFOX3/NeuN Antibody TA309832AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

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  Immunocytochemistry/Immunofluorescence: RBFOX3/NeuN Antibody TA309832 - Neuro2a cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-RBFOX3/NeuN Antibody TA309832 at 1 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Immunohistochemistry: RBFOX3/NeuN Antibody TA309832 - Analysis of NeuN in mouse brain using DAB with hematoxylin counterstain.

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  Immunohistochemistry-Paraffin: RBFOX3/NeuN Antibody TA309832 - IHC analysis of formalin fixed paraffin embedded tissue section from hippocampus of mouse brain with NeuN antibody at 1:200 dilution. The antibody generated a strong nuclear with weak cytoplasmic staining in NeuN reactive cells.

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Specifications

Product Data

• Applications: FC, ICC/IF, IHC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:50 - 1:100, Flow Cytometry, Immunohistochemistry-Frozen, Immunohistochemistry Free-Floating, Western Blot, Immunohistochemistry: 1:200, Immunohistochemistry-Paraffin: 1:200
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal region of the human RBFOX/Neun protein (within residues 20-100). [Swiss-Prot A6NFN3]
• Formulation: PBS, 30% glycerol and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RNA binding protein, fox-1 homolog 3
• Database Link:
  NP_001020619
  Entrez Gene 52897 MouseEntrez Gene 146713 Human
  UniProt ID A6NFN3
• Synonyms: Fox-3; Hrnbp3; NeuN; Neun; Neuna60; RGD1560070