POLR2A Mouse Monoclonal Antibody [Clone ID: 4H8]

CAT#: TA309814

Mouse Monoclonal RNA polymerase II Antibody (4H8)

Product Images

Immunocytochemistry/Immunofluorescence: RNA Polymerase II/POLR2A Antibody (4H8) TA309814 - RNA Polymerase II/POLR2A antibody was tested in HeLa cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

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  Flow Cytometry: RNA Polymerase II/POLR2A Antibody (4H8) TA309814 - An intracellular stain was performed on HeLa cells with RNA Polymerase II/POLR2A (4H8) antibody TA309814PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes. Both antibodies were conjugated to phycoerythrin.

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  Flow (Intracellular): RNA Polymerase II/POLR2A Antibody (4H8) TA309814 - An intracellular stain was performed on Daudi cells with RNA Polymerase II/POLR2A (4H8) antibody TA309814 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

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  Western Blot: RNA Polymerase II/POLR2A Antibody (4H8) TA309814 - Analysis of MCF7 cell lysata (A) using RNA polymerase II antibody at 2 ug/mL.

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  Western Blot: RNA Polymerase II/POLR2A Antibody (4H8) TA309814 - Whole cell protein from HeLa (lane 1), MCF7 (lane 2), NIH-3T3 (lane 3) and Cos7 (lane 4) were separated by SDS-PAGE on a 6% gel, transferred to PVDF and probed with 2 ug/mL TA309814. RNA Pol II reactivity (arrowhead) is observed in HeLa, MCF and 3T3 cells.

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Specifications

Product Data

• Clone Name: 4H8
• Applications: ChIP, CyTOF-ready, ELISA, FC, ICC/IF, IP, WB
• Recommended Dilution: Western Blot: 1-2 ug/ml, ELISA: 1:100-1:2000, Immunocytochemistry/ Immunofluorescence: 1:100-1:1000, Immunoprecipitation: 1:10-1:500, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Flow Cytometry: 1 ug per million cells, Flow (Intracellular), CyTOF-ready
• Reactivities: Human, Mouse, Yeast
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-ser 5 YSPTSpPS (Human).
• Formulation: PBS
• Concentration: lot specific
• Purification: IgG purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: polymerase (RNA) II subunit A
• Database Link:
  NP_000928
  Entrez Gene 20020 MouseEntrez Gene 5430 Human
  UniProt ID P24928
• Synonyms: hRPB220; hsRPB1; POLR2; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2

Reference Data

• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase