HIF-1 alpha (HIF1A) Mouse Monoclonal Antibody [Clone ID: ESEE122]

CAT#: TA309690

Mouse Monoclonal HIF-1 alpha Antibody (ESEE122)

Product Images

Simple Western: HIF-1 alpha Antibody (ESEE122) TA309690 - Image shows a specific band for HIF-1 alpha in 0.5 mg/mL of Hypoxic HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

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  Immunocytochemistry/Immunofluorescence: HIF-1 alpha Antibody (ESEE122) TA309690 - Detection of HIF-1 alpha (red dye) in a cell cytospin from a lavage of a murine skin pouch infected with S. aureus. 100X magnification. Blue: DAPI nuclear staining.

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  Immunohistochemistry: HIF-1 alpha Antibody (ESEE122) TA309690 - HIF-1 alpha staining in hypoxia-induced human placenta.

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  Positive immunohistochemical staining for (A) VEGF, (B) HIF-1α, (C) Dll4 (tumor cells), (D) Dll4 (endothelial cells), and (E) CD31 (for microvessel counting, ×200 magnification).

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  Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) TA309690 - Negative control stain of human placenta (from sea level) using mouse IgG at 1:100. 4uM paraffin-embedded section.

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  Immunohistochemical analysis in non-GIST STS representing negative, and score 1–3 of CAIX, GLUT-1, HIF-1α, and HIF-2α. non-GIST STS: non-gastrointestinal stromal tumor soft-tissue sarcomas, CAIX: carbonic anhydrase IX, GLUT-1: glucose transporter-1, and HIF-1/2α: hypoxia induced factor 1/2α.

• Immunohistochemistry-Paraffin: HIF-1 alpha Antibody (ESEE122) TA309690 - Analysis of a FFPE tissue section of human renal cancer xenograft using HIF-1 alpha antibody (TA309690 Lot 83115) at 1:200 dilution. The antibody generated a strong cytoplasmic staining mainly in the cancer cells. Only a fraction of cells depicted nuclear staining, while weak to negligible positivity was seen in the tumor stromal cells.

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Specifications

Product Data

• Clone Name: ESEE122
• Applications: FC, ICC/IF, IHC, Immunoblotting, IP, Simple Western, WB
• Recommended Dilution: Western Blot: 1:500-1:1000, Simple Western: 1:2000, Immunohistochemistry: 1:100-1:5000, Immunohistochemistry-Frozen: 1:100-1:5000, Immunohistochemistry-Paraffin: 1:100-1:5000, Immunoblotting, Immunoprecipitation: 1:10-1:500, Immunocytochemistry/ Immunofluorescence: 1:100, Flow Cytometry
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human HIF-1 alpha amino acids 329-530.
• Formulation: Tris-glycine, 150mM NaCl, 0.05% Sodium Azide and 0.05% Sodium Azide
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: hypoxia inducible factor 1 alpha subunit
• Database Link:
  NP_001521
  Entrez Gene 15251 MouseEntrez Gene 29560 RatEntrez Gene 3091 Human
  UniProt ID Q16665
• Synonyms: bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma