BMI1 Mouse Monoclonal Antibody [Clone ID: LLBmi1-1]

CAT#: TA309589

Mouse Monoclonal Bmi1 Antibody (LLBmi1-1)

Product Images

Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) TA309589 - Bmi1 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).

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  Flow Cytometry: BMI-1 Antibody (LLBmi1-1) TA309589 - An intracellular stain was performed on U2OS cells with BMI-1 Antibody [LLBmi1-1] TA309589AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

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  Flow Cytometry: BMI-1 Antibody (LLBmi1-1) TA309589 - An intracellular stain was performed on U2OS cells with BMI-1 [LLBmi1-1] Antibody TA309589 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).

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  Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) TA309589 - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- TA309589 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

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  Immunocytochemistry/Immunofluorescence: BMI-1 Antibody (LLBmi1-1) TA309589 - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- TA309589 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.

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  Flow Cytometry: BMI-1 Antibody (LLBmi1-1) TA309589 - An intracellular stain was performed on U937 cells with BMI-1 Antibody (LLBmi1-1) TA309589 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

• Flow Cytometry: BMI-1 Antibody (LLBmi1-1) TA309589 - An intracellular stain was performed on NIH3T3 cells with BMI-1 Antibody (LLBmi1-1) TA309589 (blue) and a matched mouse IgG2b Kappa isotype control (orange) MAB004. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).

• Western Blot: BMI-1 Antibody (LLBmi1-1) TA309589 - Western blot analysis of Bmi1 expression in 1) U2OS and 2) K562 whole cell lysates using TA309589.

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Specifications

Product Data

• Clone Name: LLBmi1-1
• Applications: ChIP, FC, ICC/IF, IP, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:100, Western Blot: 1:1000, Immunoprecipitation: 1:10-1:500, Chromatin Immunoprecipitation (ChIP): 1:10-1:500, Flow Cytometry, Flow (Intracellular)
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: Human Bmi1 protein [Swiss-Prot# P35226]
• Formulation: Tris-glycine, 150mM NaCl and 0.05% Sodium Azide
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: BMI1 proto-oncogene, polycomb ring finger
• Database Link:
  NP_005171
  Entrez Gene 12151 MouseEntrez Gene 648 Human
  UniProt ID P35226
• Synonyms: BMI1; FLVI2; PCGF4; RNF51

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors