PLK1 Rabbit Polyclonal Antibody

CAT#: TA309215

Rabbit Anti-Polo-Like Kinase (Thr210) Antibody (Phospho-Specific)

Product Images

Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 k PLK protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that it was incubated in λ-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK antibody. The immunolabeling is completely eliminated by treatment with λ-Ptase.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 1:1000
• Reactivities: Rat, Human
• Modifications: Phospho-specific
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr210 conjugated to KLH
• Formulation: 100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Purification: Affinity Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 66 kDa
• Gene Name: polo like kinase 1
• Database Link:
  NP_005021
  Entrez Gene 25515 RatEntrez Gene 5347 Human
  UniProt ID P53350
• Synonyms: PLK; STPK13

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation