NLRP3 Rabbit Polyclonal Antibody

CAT#: TA309046

Rabbit polyclonal antibody to NLRP3 (NLR family, pyrin domain containing 3)

Product Images

Sample (30 ug of whole cell lysate). A:Raji. 7.5% SDS PAGE. TA309046 diluted at 1:1500

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB:1:500-1:3000
• Reactivities: Human (Predicted: Rat, Pig, Bovine, Rhesus Monkey, Mouse)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region within amino acids 974 and 1036 of NLRP3 (Uniprot ID#Q96P20)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 118 kDa
• Gene Name: NLR family, pyrin domain containing 3
• Database Link:
  NP_001120934
  Entrez Gene 216799 MouseEntrez Gene 287362 RatEntrez Gene 114548 Human
  UniProt ID Q96P20
• Background: This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq]
• Synonyms: AGTAVPRL; AII; AVP; C1orf7; CIAS1; CLR1.1; FCAS; FCAS1; FCU; MWS; NALP3; PYPAF1
• Note: Seq homology of immunogen across species: Mouse (85%), Rat (92%), Pig (92%), Rhesus Monkey (92%), Bovine (92%)

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: NOD-like receptor signaling pathway