HNF1 alpha (HNF1A) Rabbit Polyclonal Antibody

CAT#: TA308567

Rabbit polyclonal antibody to HNF-1 alpha (HNF1 homeobox A)

Product Images

HNF1 alpha antibody [N1N3] detects HNF1 alpha protein by Western blot analysis. A. 30 ug HepG2 whole cell lysate/extract. 7.5 % SDS-PAGE. HNF1 alpha antibody [N1N3] (TA308567) dilution: 1:5000

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  Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using HNF-1 alpha (TA308567) antibody at 1:100 dilution.

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  HNF1 alpha antibody [N1N3] detects HNF1 alpha protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HNF1 alpha protein stained by HNF1 alpha antibody [N1N3] (TA308567)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:1000-1:10000
• Reactivities: Human (Predicted: Mouse, Pig, Rat, Bovine, X. tropicalis)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 35 and 268 of HNF1 alpha (Uniprot ID#P20823)
• Formulation: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 67 kDa
• Gene Name: HNF1 homeobox A
• Database Link:
  NP_000536
  Entrez Gene 21405 MouseEntrez Gene 24817 RatEntrez Gene 6927 Human
  UniProt ID P20823
• Background: The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. [provided by RefSeq]
• Synonyms: HNF-1A; HNF1; IDDM20; LFB1; MODY3; TCF-1; TCF1
• Note: Seq homology of immunogen across species: Mouse (99%), Pig (99%), Rat (99%), Bovine (98%), Xenopus tropicalis (86%)

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Maturity onset diabetes of the young