SQSTM1 Rabbit Polyclonal Antibody

CAT#: TA306861

Rabbit Polyclonal SQSTM1 Antibody

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: WB: 0.5-2 µg/mL; IF: 20 µg/mL; IHC: 2-5 µg/mL;.
  Antibody validated: Western Blot in human and mouse samples; Immunofluorescence and Immunohistochemistry in human, mouse and rat samples. All other applications and species not yet tested.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: SQSTM1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human SQSTM1. The immunogen is located within the last 50 amino acids of SQSTM1.
• Formulation: PBS containing 0.02% sodium azide.
• Purification: SQSTM1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: sequestosome 1
• Database Link:
  NP_001135770
  Entrez Gene 18412 MouseEntrez Gene 113894 RatEntrez Gene 8878 Human
  UniProt ID Q13501
• Background: SQSTM1/p62 is an adapter protein which binds ubiquitin and regulates signaling cascades through ubiquitination. It may regulate the activation of NF-kappaB by TNF-alpha, nerve growth factor (NGF) and interleukin-1. SQSTM1/p62, a co-interacting protein of the atypical PKC isoforms, has a UBA domain at its C-terminal end, which binds non-covalently to polyubiquitin chain. SQSTM1’s UBA domain is necessary for recruitment of polyubiquitin and aggresome formation. SQSTM1 may play a role in titin/TTN downstream signaling in muscle cells and may be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels. Mutations in the ubiquitin-associated (UBA) domain of SQSTM1 commonly cause Paget’s disease of bone since the UBA is necessary for aggregate sequestration and cell survival.
• Synonyms: A170; OSIL; p60; p62; p62B; PDB3; ZIP3

Reference Data

• Protein Families: Druggable Genome, Transcription Factors