Niemann Pick C1 (NPC1) Rabbit Polyclonal Antibody

CAT#: TA306785

Rabbit Polyclonal NPC1 Antibody

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: NPC1 antibody can be used for detection of NPC1 by Western blot at 1 µg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 µg/mL. For immunofluorescence start at 20 µg/mL.
  Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: NPC1 antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human NPC1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: NPC1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: NPC intracellular cholesterol transporter 1
• Database Link:
  NP_000262
  Entrez Gene 18145 MouseEntrez Gene 4864 Human
  UniProt ID O15118
• Background: Mutations in the Niemann-Pick disease type C1 (NPC1) gene result in a fatal progressive neurodegenerative disorder characterized by an abnormal sequestration of lipids including cholesterol and glycosphingolipids. The NPC1 protein is a large protein that resides in the limiting membrane of endosomes and lysosomes and mediates intracellular cholesterol trafficking via binding of cholesterol to its N-terminal domain. NPC1 transports low-density lipoproteins to late endosomal/lysosomal compartments where they are hydrolized and released as free cholesterol. NPC1, in addition to FTO, MC4R, and PTER has recently been shown to be a new risk loci for early-onset and morbid adult obesity in European populations. This anti-NPC1 antibody will not cross-react to NPC2, another gene whose defects also result in Niemann-Pick type C disease.
• Synonyms: NPC

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Lysosome