FBXL20 Rabbit Polyclonal Antibody

CAT#: TA306519

Rabbit Polyclonal SCRAPPER Antibody

Product Images

Western blot analysis of SCRAPPER in A20 cell lysate with SCRAPPER antibody at (A) 0.5 and (B) 1 ug/ml.

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  Immunohistochemical staining of human brain tissue using Scrapper antibody at 2.5 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL, ICC: 2.5 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: SCRAPPER antibody was raised against a 18 amino acid peptide near the carboxy terminus of the human SCRAPPER.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: F-box and leucine-rich repeat protein 20
• Database Link:
  NP_116264
  Entrez Gene 84961 Human
  UniProt ID Q96IG2
• Background: Members of the F-box protein family, such as Scrapper, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1, cullin, and F-box proteins, act as protein-ubiquitin ligases. Scrapper is selectively expressed in the brain, broadly expressed within the mouse CNS and is abundant at presynaptic membrane. Scrapper has orthologs in C. elegans, D. melanogaster, and mammals which suggests that it might function as an important membrane-localized E3 ligase in various species. Scrapper is a major presynaptic E3 ubiquitin ligase that acts through RIM1a via degradation and the ubiquitin-proteasome-system (UPS) pathway to critically regulate synaptic transmission. This identifies protein degradation as a mechanism for holding synaptic communication in check.
• Synonyms: Fbl2; Fbl20