AIRE Goat Polyclonal Antibody

CAT#: TA302805

Goat Polyclonal Antibody against AIRE (C-terminal)

Product Images

TA302805 (0.5ug/ml) staining of Human Lymph lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TA302805 (2ug/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1:128,000; WB: 0.5-1.5 ug/ml; IHC: 2.5µg/ml.
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-QSMARPAAPFPS, from the C Terminus of the protein sequence according to NP_000374.1, NP_000649.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: autoimmune regulator
• Database Link:
  NP_000374
  Entrez Gene 326 Human
  UniProt ID O43918
• Background: This gene encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED). [provided by RefSeq]
• Synonyms: AIRE1; APECED; APS1; APSI; PGA1
• Note: In paraffin embedded Human Thymic medula shows staining of select nuclei. A customer has reported this antibody was successful in ChIP.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Primary immunodeficiency, Ubiquitin mediated proteolysis