PCK2 Goat Polyclonal Antibody

CAT#: TA302563

Goat Polyclonal Antibody against PCK2 / PEPCK-M

Product Images

TA302563 (2 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 2-4 µg/ml.
• Reactivities: Human, Mouse, Rat, Guinea Pig (Expected from sequence similarity: Dog, Pig, Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence KPWKPGDKEPCAH, from the internal region of the protein sequence according to NP_004554.2.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 74360 Da
• Gene Name: phosphoenolpyruvate carboxykinase 2, mitochondrial
• Database Link:
  NP_004554
  Entrez Gene 74551 MouseEntrez Gene 361042 RatEntrez Gene 480255 DogEntrez Gene 5106 Human
  UniProt ID Q16822
• Background: This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq]
• Synonyms: PEPCK; PEPCK-M; PEPCK2

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Adipocytokine signaling pathway, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism