BHMT Goat Polyclonal Antibody

CAT#: TA302433

Goat Polyclonal Antibody against BHMT

Product Images

TA302433 (0.03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  HEK293 overexpressing BHMT RC203148) and probed with TA302433 (mock transfection in first lane)

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1:16,000. WB: 0.03-0.1µg/ml.
• Reactivities: Human, Mouse, Rat (Expected from sequence similarity: Dog)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-EQQLKELFEKQK, from the C Terminus of the protein sequence according to NP_001704.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48625 Da
• Gene Name: betaine--homocysteine S-methyltransferase
• Database Link:
  NP_001704
  Entrez Gene 12116 MouseEntrez Gene 81508 RatEntrez Gene 479171 DogEntrez Gene 635 Human
  UniProt ID Q93088
• Background: This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq]
• Synonyms: BHMT1; HEL-S-61p

Reference Data

• Protein Pathways: Cysteine and methionine metabolism, Glycine, serine and threonine metabolism, Metabolic pathways