GATA4 Rabbit Polyclonal Antibody

CAT#: TA302164

Rabbit Polyclonal Antibody against GATA4 (C-term)

Product Images

Western blot analysis of anti-GATA4 Antibody (C-term) (Cat.#TA302164) in CEM cell line lysates (35ug/lane). GATA4 (arrow) was detected using the purified Pab.

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  Western blot analysis of GATA4 (arrow) using rabbit polyclonal GATA4 Antibody (C-term) (Cat.#TA302164). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GATA4 gene (Lane 2) (Origene Technologies).

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  GATA4 Antibody (C-term) (Cat. #TA302164) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: WB: 1:1000, FC: 1:10~50
• Reactivities: Human (Predicted: Mouse, Rat, Xenopus)
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This GATA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-328 amino acids from the C-terminal region of human GATA4.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44627 Da
• Gene Name: GATA binding protein 4
• Database Link:
  NP_002043
  Entrez Gene 14463 MouseEntrez Gene 54254 RatEntrez Gene 2626 Human
  UniProt ID P43694
• Background: GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in the gene encoding GATA4 have been associated with cardiac septal defects.
• Synonyms: ASD2; TACHD; TOF; VSD1

Reference Data

• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors