SAE1 Rabbit Polyclonal Antibody

CAT#: TA302053

Rabbit Polyclonal Antibody against SAE1 (C-term)

Product Images

The AOS1 C-term Pab (Cat. #TA302053) is used in Western blot to detect AOS1 in mouse heart tissue lysate.

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  Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term) (Cat.#TA302053). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOS1 gene (Lane 2) (Origene Technologies).

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  AOS1 Antibody (V315) (Cat. #TA302053) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AOS1 antibody detected the AOS1 protein (arrow).

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:50~100
• Reactivities: Human, Mouse (Predicted: Rat, Bovine)
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 amino acids from the C-terminal region of human SAE1 (AOS1).
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 38450 Da
• Gene Name: SUMO1 activating enzyme subunit 1
• Database Link:
  NP_005491
  Entrez Gene 56459 MouseEntrez Gene 308384 RatEntrez Gene 10055 Human
  UniProt ID Q9UBE0
• Background: The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.
• Synonyms: AOS1; HSPC140; SUA1; UBLE1A

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis