ATP7B Rabbit Polyclonal Antibody

CAT#: TA301761

Rabbit Polyclonal Antibody against ATP7b

Product Images

Immunocytochemistry/Immunofluorescence: ATP7b Antibody TA301761 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-ATP7b TA301761 at a 1:100 dilution overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Immunohistochemistry: ATP7b Antibody TA301761 - Analysis of ATP7b in mouse liver using DAB with hematoxylin counterstain.

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  Western Blot: ATP7b Antibody TA301761 - ATP7b detected in 20 ug of mouse brain membrane fraction.

Specifications

Product Data

• Applications: ICC/IF, IHC, Immunoblotting, IP, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 2 - 10 ug/mL, Immunohistochemistry: 1:100 - 1:500, Immunohistochemistry-Paraffin: 1:100 - 1:500, Immunoprecipitation, Western Blot: 1:1000, Immunoblotting
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal sequence near the N-terminus of human ATP7b.
• Formulation: Tris citrate/phosphate [pH 7-8] and 0.1% sodium azide
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ATPase copper transporting beta
• Database Link:
  NP_000044
  Entrez Gene 11979 MouseEntrez Gene 540 Human
  UniProt ID P35670
• Background: ATP7b is an important protein for copper transport and elimination of excess copper from the body. ATP7b transports metals in and out of cells using ATP. There are 3 known isoforms of the ATP7b gene; A is found in the liver, kidney, and brain, the shorter form B is found in brain tissue, and the third isoform, known as WND/140 KDA is found in mitochondria. Mutations in the ATP7b gene can cause Wilson's disease, an inherited disorder causing copper poisoning in the brain and liver.
• Synonyms: PWD; WC1; WD; WND

Reference Data

• Protein Families: Druggable Genome, Transmembrane