HIF1AN Rabbit Polyclonal Antibody

CAT#: TA301533

Rabbit Polyclonal Antibody against Factor Inhibiting HIF-1

Product Images

Western Blot: FIH-1/HIF-1AN Antibody TA301533 - Analysis of astrocytes lysate from Rat brain using FIH-1/HIF-1AN antibody at 1:500 dilution with ECL detection. The antibody detected a major band at ~40 kDa position representing FIH-1/HIF-1AN protein.

• 

  Immunohistochemistry: FIH-1/HIF-1AN Antibody TA301533 - Detection of FIH-1 in rat brain tissue.

Specifications

Product Data

• Applications: Electron Microscopy, ICC/IF, IHC, IP, WB
• Recommended Dilution: Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation: 1:10-1:500, Electron Microscopy, Immunohistochemistry-Paraffin: 1:50-1:200, Immunohistochemistry-Frozen: 1:50-1:200, Western Blot: 1:1000-1:5000, Knockdown Validated, Knockout Validated
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A C-terminal region synthetic peptide made to the human FIH protein sequence (between residues 300 and the C-terminus).
• Formulation: Tris-citrate/phosphate [pH 7-8] buffer. and 0.1% Sodium Azide
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: hypoxia inducible factor 1 alpha subunit inhibitor
• Database Link:
  NP_060372
  Entrez Gene 309434 RatEntrez Gene 55662 Human
  UniProt ID Q9NWT6
• Background: Factor Inhibiting HIF1 (FIH) protein facilitates repression of HIF-1 transcriptional activity by binding to VHL, which acts as a transcriptional corepressor. VHL inhibits HIF-1 alpha transactivation function by recruiting histone deacetylases. Involvement of VHL in association with FIH provides a unifying mechanism for the modulation of HIF-1 alpha protein stabilization and transcriptional activation in response to changes in cellular oxygen concentration.
• Synonyms: FIH1

Reference Data

• Protein Families: Druggable Genome