CD9 Mouse Monoclonal Antibody [Clone ID: MEM-61]

CAT#: SM3039P

CD9 mouse monoclonal antibody, clone MEM-61, Purified

Product Images

Surface staining of NALM-6 human pre-B cell leukemia cell line with anti-human CD9 (MEM-61) FITC. Total viable cells were used for analysis.

Specifications

Product Data

• Clone Name: MEM-61
• Applications: FC, FN, IHC, IP, WB
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 20 μg/ml.
  Positive tissue: Prostate.
  Flow Cytometry: 5 μg/ml.
  Immunoprecipitation.
  Western blot: 2-4 µg/ml (Non Reducing Conditions).
  Functional Application: The antibody induces FcgR-dependent platelet activation / aggregation.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Pre-B cell line NALM-6
• Specificity: The antibody recognizes an epitope on second extracellular domain (EC2) of CD9 antigen, a 24 kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
• Formulation: Phosphate buffered saline (PBS), pH~7.4 with 15 mM Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fractionn (> 95% pure by SDS-PAGE)
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: CD9 molecule
• Database Link:
  Entrez Gene 928 Human
  UniProt ID P21926
• Background: CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
• Synonyms: MIC3, TSPAN29, GIG2, p24, Tetraspanin-29, 5H9 antigen