CD11b Mouse Monoclonal Antibody [Clone ID: OX-42]

CAT#: SM262PS

CD11b mouse monoclonal antibody, clone OX-42, Purified

Product Images

Staining of Rat peritoneal macrophages with Mouse anti Rat CD11b Antibody (Cat.-No SM262P)

Specifications

Product Data

• Clone Name: OX-42
• Applications: FC, FN, IHC, IP
• Recommended Dilution: Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10⁶ cells in 100 µl.
  Immunohistochemistry on Frozen Sections: 1/50-1/100.
  Immunoprecipitation.
  Immunofluorescence.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Rat peritoneal macrophages.
  Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
• Specificity: This antibody recognises the equivalent of Human CD11b, the receptor for the iC3b component of complement. The antibody also labels dendritic cells, granulocytes and microglial cells in the brain (Robinson et al., 1986).
  Clone MRC OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).
  Functionally, clone OX-42 inhibits complement mediated rosettes (Robinson et al., 1986) as well as inhibit myelin binding and uptake (van der Laan et al., 1996). We recommend the use of SM262LE for use in functional studies.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID Q63001
• Background: The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages.
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1