CD11b Mouse Monoclonal Antibody [Clone ID: OX-42]

CAT#: SM262P

CD11b mouse monoclonal antibody, clone OX-42, Purified

Size: 100 ug 250 ug

Conjugation: Unconjugated Biotin FITC


USD 569.00

5 Days*

Size
    • 250 ug

Product Images

Specifications

Product Data
Clone Name OX-42
Applications FC, FN, IF, IHC, IP
Recommended Dilution Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl.
Immunoprecipitation. 
Immunofluorescence.

Immunohistochemistry on Frozen Sections:
1/50-1/100.
Immunohistochemistry on Paraffin Sections: Clone OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). See Whiteland, J.L. et al. for details.
Functionally Assays: Clone OX-42 inhibits complement mediated rosettes. We recommend the use of Low Endotoxin CD11b Antibody Cat.-No SM262LE for use in functional studies. See Robinson, A.P. et al. for details.
Reactivities Rat
Host Mouse
Isotype IgG2a
Clonality Monoclonal
Immunogen Rat peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the NSO/U mouse myeloma cell line.
Specificity This antibody recognizes the Rat equivalent of human CD11b, the receptor for the iC3b component of complement.
The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages.
The antibody also labels dendritic cells, granulocytes and microglial cells in the brain.
Formulation PBS, pH 7.2
State: Purified
State: Liquid purified IgG fraction
Preservative: 0.09% Sodium Azide
Concentration lot specific
Purification Affinity Chromatography on Protein G of Tissue Culture Supernatant
Conjugation Unconjugated
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue.
Synonyms ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data

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