CD11b Mouse Monoclonal Antibody [Clone ID: OX-42]

CAT#: SM262F

CD11b mouse monoclonal antibody, clone OX-42, FITC

Specifications

Product Data

• Clone Name: OX-42
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10µl of neat - 1/10 diluted antibody to label 10e6 cells in 100µl.
  Clone OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Functionally, clone OX-42 inhibits complement mediated rosettes. We recommend the use of SM262LE for use in functional studies.
• Reactivities: Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Rat resident peritoneal macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSO/U myeloma cell line.
• Specificity: This antibody recognises the equivalent of human CD11b, the receptor for the iC3b component of complement. The antibody also labels dendritic cells, granulocytes and microglial cells in the brain.
• Formulation: Containing 0.09% Sodium Azide and 1% Bovine Serum Albumin
  Label: FITC
  State: Liquid IgG fraction
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity chromatography on protein G
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID Q63001
• Background: The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages.
• Synonyms: ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1