CD14 Mouse Monoclonal Antibody [Clone ID: Tük4]

CAT#: SM1066F

CD14 mouse monoclonal antibody, clone Tük4, FITC

Product Images

Staining of human peripheral blood monocytes probed with anti-CD14:FITC

Specifications

Product Data

• Clone Name: Tük4
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10⁶ cells or 100 µl whole blood.
• Reactivities: Bovine, Canine, Feline, Goat, Human, Mink, Monkey, Porcine, Rabbit, Sheep, Llama
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: CD14 Human
• Specificity: This antibody recognises the CD14 cell surface antigen. CD14 is expressed strongly by most peripheral blood monocytes and weakly on granulocytes.
  Clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose-dependent manner (6). SM1066LE is recommended for this purpose.
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD14 molecule
• Database Link:
  Entrez Gene 929 Human
  UniProt ID P08571
• Background: CD14 (also known lipopolysaccharide (LPS) receptor) is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
• Synonyms: CD14