CD14 Mouse Monoclonal Antibody [Clone ID: Tük4]

CAT#: SM1066P

CD14 mouse monoclonal antibody, clone Tük4, Purified

Product Images

Staining of human peripheral blood monocytes probed with Mouse anti Human CD14 (SM1066P)

Specifications

Product Data

• Clone Name: Tük4
• Applications: ELISA, FC, WB
• Recommended Dilution: ELISA.
  Western Blot (Reducing Conditions).
  Flow Cytometry: Use 5 µl of 1/50-1/200 diluted antibody to label 10e6 cells or 100 µl whole blood.
• Reactivities: Bovine, Canine, Feline, Goat, Human, Mink, Monkey, Porcine, Rabbit, Sheep
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Specificity: SM1066P recognises the CD14 cell surface antigen, expressed strongly by most peripheral blood monocytes and weakly on granulocytes.
  Clone TÜK4 has been shown to block SDF-induced chemotaxis of U937 cells in a dose-dependent manner (6). We recommend the use of SM1066LE for this purpose.
• Formulation: PBS containing 0.09% Sodium Azide as preservative.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD14 molecule
• Database Link:
  Entrez Gene 929 Human
  UniProt ID P08571
• Background: CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidyl-inositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.
• Synonyms: CD14