Macrophage inflammatory protein 5 (CCL15) Goat Polyclonal Antibody

CAT#: PP1088P2

Macrophage inflammatory protein 5 (CCL15) goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-5 (100.00 ng/ml), a concentration of 2.0-3.0 µg/ml of this antibody is required.
  ELISA: To detect hMIP-5 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant hMIP-5.
  Western Blot: To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant hMIP-5.
• Specificity: Macrophage Inflammatory Protein-5 (MIP-5).
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: C-C motif chemokine ligand 15
• Database Link:
  Entrez Gene 6359 Human
  UniProt ID Q16663
• Background: Macrophage inflammatory protein 5 (CCL15) is a member of the human beta-chemokine family and is chemotactic for T cells and monocytes but not neutrophils, eosinophils, or B cells. It induces changes in intracellular calcium concentration in monocytes and is thought to act through the CCR1 receptor but also binds to CCR3.
• Synonyms: C-C motif chemokine 15, Small-inducible cytokine A15, Chemokine CC-2, NCC-3, NCC3, MIP-5, MIP-1 delta, Leukotactin-1, Mrp-2b, SCYA15
• Note: Centrifuge vial prior to opening!