Csf3 Rabbit Polyclonal Antibody

CAT#: PP022B1

Csf3 rabbit polyclonal antibody, Biotin

Product Images

Direct ELISA using G-CSF Antibody Cat.-No PP022B

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  Sandwich ELISA using G-CSF Antibody Cat.-No PP022B

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Mouse G-CSF by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1.0 µg/ml. This Biotin conjugated antibody allows the detection of at least 0.2 ng/well of recombinant mG-CSF.
  Sandwich ELISA: To detect Mouse G-CSF by Sandwich ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. Used in conjunction with Purified Anti- Murine G-CSF (Cat.-No PP022P1 or PP022P2), allows the detection of at least 0.2-0.4 ng/well of recombinant Mouse G-CSF.
  Western blot: To detect Mouse G-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Mouse G-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Murine G-CSF.
• Specificity: This antibody reacts with Murine G-CSF.
• Formulation: PBS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction
  Label: conjugated
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: colony stimulating factor 3 (granulocyte)
• Database Link:
  Entrez Gene 12985 Mouse
  UniProt ID P09920
• Background: Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.
• Synonyms: CSF3, GCSF, Pluripoietin, C17orf33
• Note: Centrifuge vial prior to opening!