Csf3 Rabbit Polyclonal Antibody

CAT#: PP022P1

Csf3 rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, FN, WB
• Recommended Dilution: Neutralization:
  To yield one-half maximal inhibition [ND50] of the biological activity of mG-CSF (0.5ng/ml), a concentration of 0.005-0.010 µg/ml of this antibody is required.
  ELISA:
  (Indirect): To detect mG-CSF by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mG-CSF.
  (Sandwich): To detect mG-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine G-CSF (PP022B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mG-CSF.
  Western Blot:
  To detect mG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) recombinant mG-CSF
• Specificity: This antibody recognises Murine G-CSF.
• Formulation: PBS, pH 7.2 without preservatives.
  State: Aff - Purified
  State: Lyophilized purified Ig fraction.
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: colony stimulating factor 3 (granulocyte)
• Database Link:
  Entrez Gene 12985 Mouse
  UniProt ID P09920
• Background: Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils.
• Synonyms: CSF3, GCSF, Pluripoietin, C17orf33
• Note: Centrifuge vial prior to opening!