Carbonic Anhydrase IX (CA9) Mouse Monoclonal Antibody [Clone ID: OTI1G7]

CAT#: CF500623

Carrier-free (BSA/glycerol-free) CA9 mouse monoclonal antibody, clone OTI1G7 (formerly 1G7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CA9 (Cat# RC204839, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CA9(Cat# TA500623). Positive lysates LY400485 (100ug) and LC400485 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-CA9mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA500623, Dilution 1:50)

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  HEK293T cells transfected with either pCMV6-ENTRY CA9 (RC204839) (Red) or empty vector control plasmid (Blue) were immunostained with anti-CA9 mouse monoclonal (TA500623, Dilution 1:1,000), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI1G7
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:50, Flow 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Full-length protein expressed in 293T cell transfected with human CA9 expression vector
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 49.7 kDa
• Gene Name: carbonic anhydrase 9
• Database Link:
  NP_001207
  Entrez Gene 768 Human
  UniProt ID Q16790
• Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-basebalance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12.
• Synonyms: CAIX; MN

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Nitrogen metabolism