alpha 1 Antitrypsin (SERPINA1) Rabbit Polyclonal Antibody

CAT#: AP08131PU-N

alpha 1 Antitrypsin (SERPINA1) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 using streptavidin~biotin system or polymer system.
  FFPE tissue sections require antigen retriever (boiling tissue in 10 mM citrate pH 6.0 for 10-20 minutes, followed by cooling at RT for 15-20 minutes).
  Recommended Positive Control: Human tonsil, liver.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified human ATT.
• Specificity: This antiboydy is specific for Alpha-1-Antitrypsin (AAT).
  Cellular Localization: Cytoplasmic staining of macrophages.
• Formulation: PBS, pH 7.4 containing 0.05% Sodium Azide as preservative and 1% BSA as stabilizer.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: serpin family A member 1
• Database Link:
  Entrez Gene 5265 Human
  UniProt ID P01009
• Background: Alpha-1-Antitrypsin (AAT) is a 51 kDa glycoprotein synthesized in the liver. It is a serum protease inhibitor in neutrophil elastase, collagenase and chymotrypsin. This protein is a serum trypsin inhibitor, the correct name is alpha-1-proteinase inhibitor (A1PI). Disorders of this enzyme include, deficiency, in a hereditary disorder, this causes degradation of lung tissue and causes pulmonary emphysema, it also causes chronic liver disease and hepatocarcinoma. AAT is present in normal liver cells, histocytes, monocytes and a variety of epithelial and mesenchymal tumors. Increase in serum levels of AAT is also reported in rheumatoid arthritis, bacterial infection, vasculistis and carcinomatosis.
• Synonyms: SERPINA1, AAT