CD79A (208-222) Mouse Monoclonal Antibody [Clone ID: HM47]

CAT#: AM26759AC-N

CD79A (208-222) mouse monoclonal antibody, clone HM47, APC

Product Images

Surface staining of CD79a in human peripheral blood with anti-CD79a (HM47) APC.

Specifications

Product Data

• Clone Name: HM47
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (1 ml) is sufficient for 100 tests.
• Reactivities: Bovine, Canine, Chicken, Equine, Guinea Pig, Human, Mouse, Porcine, Primate, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a
• Specificity: This antibody reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.
• Formulation: Phosphate buffered saline (PBS)
  Label: APC
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
  Label: Conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: APC
• Storage: Store undiluted the antibody at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD79a molecule
• Database Link:
  Entrez Gene 973 Human
  UniProt ID P11912
• Background: CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
• Synonyms: IGA, MB1, B-Cell marker