CD79A (208-222) Mouse Monoclonal Antibody [Clone ID: HM47]

CAT#: AM26759PU-N

CD79A (208-222) mouse monoclonal antibody, clone HM47, Purified

Product Images

Surface staining of CD79a in human peripheral blood with anti-CD79a (HM47) APC.

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  Surface staining of CD79a in human peripheral blood with anti-CD79a (HM47) PE.

Specifications

Product Data

• Clone Name: HM47
• Applications: FC, IHC, IP, WB
• Recommended Dilution: Flow cytometry: 1-4 μg/ml. Intracellular staining.
  Immunoprecipitation.
  Western blot: 1-2 μg/ml.
  Immunohistochemistry (paraffin sections).
• Reactivities: Bovine, Canine, Chicken, Equine, Guinea Pig, Human, Mouse, Porcine, Primate, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to C terminal amino acids 208-222 of human CD79a
• Specificity: This antibody reacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.
• Formulation: Phosphate buffered saline (PBS)
  State: Purified
  State: Liquid Ig fraction
  Preservative: 15 mM sodium azide, approx. pH 7.4
• Concentration: lot specific
• Purification: Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: CD79a molecule
• Database Link:
  Entrez Gene 973 Human
  UniProt ID P11912
• Background: CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
• Synonyms: IGA, MB1, B-Cell marker