CD62P (SELP) Mouse Monoclonal Antibody [Clone ID: AK4]

CAT#: AM26020FC-N

CD62P (SELP) mouse monoclonal antibody, clone AK4, FITC

Specifications

Product Data

• Clone Name: AK4
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10e6 cells in a suspension.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human platelets
• Specificity: This antibody recognizes CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.
• Formulation: Phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent
  Label: FITC
  State: Liquid purified Ig fraction
  Label: Conjugated with Fluorescein isothiocyanate under optimum conditions. The reagent is free of unconjugated and adjusted for direct use
• Conjugation: FITC
• Storage: Store the antibody at 2 - 8 °C. DO NOT FREEZE! This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: selectin P
• Database Link:
  Entrez Gene 6403 Human
  UniProt ID P16109
• Background: CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
• Synonyms: SELP, GMRP, GRMP, PADGEM, GMP-140, LECAM3

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs)