CD62P (SELP) Mouse Monoclonal Antibody [Clone ID: AK4]

CAT#: AM26020PU-N

CD62P (SELP) mouse monoclonal antibody, clone AK4, Aff - Purified

Specifications

Product Data

• Clone Name: AK4
• Applications: FC, FN, IF, IP, WB
• Recommended Dilution: Flow cytometry: 1.5 µg/ml.
  Immunoprecipitation.
  Western blot.
  Immunocytochemistry.
  Functional application: Blocking.
• Reactivities: Human, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human platelets
• Specificity: This antibody recognizes CD62P (P-selectin), a 140 kD single chain type I transmembrane glycoprotein present in secretory alpha-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.
• Formulation: Phosphate buffered saline (PBS)
  State: Aff - Purified
  State: Liquid Ig fraction
  Preservative: 15 mM sodium azide, approx. pH 7.4
• Concentration: lot specific
• Purification: Protein-A affinity chromatography
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: selectin P
• Database Link:
  Entrez Gene 6403 Human
  UniProt ID P16109
• Background: CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.
• Synonyms: SELP, GMRP, GRMP, PADGEM, GMP-140, LECAM3