CD24 Mouse Monoclonal Antibody [Clone ID: SN3]

CAT#: AM03032RP-N

CD24 mouse monoclonal antibody, clone SN3, PE

Product Images

Surface staining of human peripheral blood cells with anti-human CD24 (SN3) purified, GAM-APC.

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  Surface staining of human peripheral blood cells with anti-human CD24 (SN3) APC-Cy7.

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  Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD24 (SN3) Nd145.

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  Surface staining of human peripheral blood cells with anti-CD24 (SN3) Alexa Fluor® 700.

Specifications

Product Data

• Clone Name: SN3
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of Human blood cells using 20 ?l reagent / 100 ?l of whole blood or 10⁶ cells in a suspension.
  The content of a vial (2 ml) is sufficient for 100 tests.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Glycoproteins purified from Human NALM-1 cell line
• Specificity: The antibody SN3 reacts with CD24, a 35-45 kDa heavily glycosylated cell surface antigen. CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cell malignancies. It is not expressed on Human thymocytes.
• Formulation: PBS
  Label: PE
  State: Liquid purified Ig fraction
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
• Conjugation: PE
• Storage: Store the antibody in the dark at 2-8°C.
  Do Not Freeze!.
  Avoid prolonged exposure to light.
• Stability: Shelf life: One year from despatch.
• Gene Name: CD24 molecule
• Database Link:
  Entrez Gene 100133941 Human
  UniProt ID P25063
• Background: CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4; it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.
• Synonyms: X62 heat stable antigen, HSA