Antibodies

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Human IgE (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to identify and measure IgE in Human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80, depending on the method used.
Reactivities Human
Conjugation FITC
Immunogen Pools of purified homogenous IgE isolated from Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgE (Fc specific) goat polyclonal antibody, HRP

Applications Enzyme-Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
To demonstrate circulating IgE antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using an reference antibody of Human origin known to be of the IgE isotype in the middle layer of the indirect test procedure.
In non-isotopic assay methodology (e.g. ELISA) to measure IgE in Human serum or other body fluids.
Recommneded Dilutions:
Histochemistry and Cytochemistry: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/25,000.
Reactivities Human
Conjugation HRP
Immunogen Purified monoclonal IgE isolated from Human serum.
Feund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgA (Secretory component) goat polyclonal antibody, FITC

Applications Can be used as reagent for the direct detection of secretory component in human cells, tissues and body fluids in Immunofluorescence, as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA).
This immunoconjugate is not pre-diluted and the optimum working dilution should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working Dilutions: 1/20-1/80.
Reactivities Human
Conjugation FITC
Immunogen Purified free Human Secretory component isolated from pooled milk is used for immunization.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgD (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology.
In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgD in human serum or other body fluid.
As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/10,000.
Performance testing. Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of human cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Human
Conjugation Biotin
Immunogen Highly purified monoclonal IgD isolated from Human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgD (Fc specific) goat polyclonal antibody, HRP

Applications Can be used in Enzyme-immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgD antibodies in serodiagnostic microbiology and autoimmune diseases; in nonisotopic assay methodology (e.g. ELISA) to measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions:
Histochemical and Cytochemical: 1/50 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/10,000.
Reactivities Human
Conjugation HRP
Immunogen Purified polyclonal IgD isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgD (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgD at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgD in human serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40, depending on the method used.
Reactivities Human
Conjugation FITC
Immunogen Pools of purified homogenous IgD isolated from human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgD (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used in direct Immunofluorescence staining of cytoplasmic IgD of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Working dilutions: 1/10 - 1/30.
Reactivities Human
Conjugation TRITC
Immunogen Purified monoclonal IgD isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgE (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled secondary antibody for Indirect detection of IgE in Human cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in human serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of human cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Human
Conjugation Unconjugated
Immunogen Purified homogenous IgE isolated from Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgE (Fc specific) goat polyclonal antibody, TRITC

Applications Can be used for direct Immunofluorescence staining of cytoplasmic IgE of appropriately treated cell and tissue substrates; to demonstrate immunoglobulins or specific antibodies in cells and tissues; to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/30.
Reactivities Human
Conjugation TRITC
Immunogen Purified monoclonal IgE isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgG (H+L chain) chicken polyclonal antibody, Aff - Purified

Applications ELISA.
Western Blot (1/1000).
Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose.
Reactivities Human
Conjugation Unconjugated
Immunogen Purified, non-denatured Human IgG (containing both heavy and light chains).
Production. Eggs were collected from hyperimmunized hens and the IgY fraction purified from the yolks. Anti-Human IgG's were affinity-purified over a column conjugated with purified Human IgG (containing both heavy and light chains).

Human IgG (F(ab)2 specific) goat polyclonal antibody, FITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/20-1/80. 
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation FITC
Immunogen Purified Fab prepared from normal IgG isolated from pooled Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgG (F(ab)2 specific) goat polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig
bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity.
This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC
Immunogen Purified Fab prepared from normal IgG isolated from pooled Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgA (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgA. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC
Immunogen Purified IgA prepared from pooled Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgG (Fc specific) sheep polyclonal antibody, TRITC

Applications Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgG. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.
Recommended Dilutions: 1/10-1/40
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Reactivities Human
Conjugation TRITC
Immunogen Purified IgG prepared from pooled Human serum.
Freund’s complete adjuvant is used in the first step of the immunization procedure.

Human IgG rabbit polyclonal antibody, Ig Fraction

Applications Immunoprecipitation.
Immunodiffusion.
Reactivities Human
Conjugation Unconjugated