Antibodies

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Peroxidase Conjugated Affinity Purified Goat anti-Mouse IgG [H&L]

Applications WB: 1: 1,000; ICC: 1:200; IP: 1:200
Reactivities Mouse IgG
Conjugation HRP

Rabbit IgG (H+L chain) goat polyclonal antibody, HRP, Lyophilized

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/150,000-1/250,000.
Immunohistochemistry: 1/500-1/2,500.
Western blot: 1/10,000-1/40,000.
Reactivities Rabbit
Conjugation HRP

GFP (Ads. to Hu, Ms, Rt Serum Proteins) goat polyclonal antibody, HRP

Applications ELISA, IHC, WB
Conjugation HRP

Human IgG (Fc specific) goat polyclonal antibody, HRP

Applications ELISA: 1/3000.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation HRP

Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000. 
Western Blot: 1/2,000-1/10,000.
Immunohistochemistry: 1/1,000-1/5,000.
Note: This product has been assayed against 1.0 µg of Rabbit IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/40,000 of the reconstitution concentration is suggested for this product.
Reactivities Rabbit
Conjugation HRP

Mouse IgE (Fc specific) goat polyclonal antibody, HRP

Applications In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgE antibodies in mouse serum or other body fluids; in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
depending on the method used.
Reactivities Mouse
Conjugation HRP

Human IgM (Mu chain specific) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions
ELISA: 1/80,000.
Western blot: 1/2,000-1/20,000.
Immunohistochemistry: 1/500-1/2,500.
Reactivities Human
Conjugation HRP

Monkey IgG (Fc specific) goat polyclonal antibody, HRP

Applications Dot blot.
Immunoblotting. 
ELISA.
Immunocytochemistry.
Immunohistochemistry on Paraffin Sections.
This antibody can be used:
In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases.
To identify a specific antigen using a reference antibody of monkey origin known to be of the IgG isotype in the middle layer of the indirect test procedure
In non-isotopic assay methodology (e.g. ELISA) to measure IgG in monkey serum or other body fluids.
This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histo- and Cytochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/5000-1/20000.
Reactivities Monkey
Conjugation HRP

Monkey IgA (Secretory component) goat polyclonal antibody, HRP

Applications Tested in immunoelectrophoresis and double radial immunodiffusion against a panel of appropriate secretions and purified immunoglobulin isotypes.
In enzyme-immunocytochemical and immunohistochemical staining of free or bound secretory component at the cellular and subcellular level. In non-isotopic assay methodology (e.g. ELISA, Western blotting) in monkey milk or other secretions. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working dilutions:
Histochemical and cytochemical 1/150 - 1/500.
ELISA and comparable non-precipitating antibody-binding assays 1/200 -1/4000.
Reactivities Chimpanzee, Monkey
Conjugation HRP

Human IgA + IgG + IgM goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/20,000-1/100,000.
Western blot: 1/2,000-1/10,000.
Immunoprecipitation: 1/1,000-1/5,000.
Reactivities Human
Conjugation HRP

FITC goat polyclonal antibody, HRP

Applications ELISA, IHC, WB
Conjugation HRP

Concanavalin-A goat polyclonal antibody, HRP

Applications ID, IF, IHC
Reactivities Jack Bean
Conjugation HRP

Rat IgA + IgG + IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1/100,000.
Western blot: 1/500-1/3,000.
Immunohistochemistry: 1/500-1/1,500.
Reactivities Rat
Conjugation HRP

Human IgE goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Human, Monkey
Conjugation HRP

Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, HRP

Applications ELISA (to identify and measure a specific IgG in rabbit serum or other body fluids).
Immunocytochemistry.
Immunohistochemistry.
Useful in electron microscopy, since the complex between the conjugated antibody and the antigen has electron-dense properties.
Dot blot.
Western blot.
General Recommended Dilutions:
Histochemical and Cytochemical Use: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Working dilutions should be prepared by adding sterile PBS, pH 7.2, and should not be refrozen.
Reactivities Rabbit
Conjugation HRP