Secondary Antibodies

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mIgG (1,2a,2b,3) Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR9F11

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse, Rat
Conjugation Unconjugated

mIgG1 Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR8A7

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

mIgG2a Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR6C3

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

mIgG3 Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR8B8

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

mIgG2a and mIgG2b Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR9H1

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

mIgG2a Fcγ Fragment Specific Rabbit monoclonal antibody,clone OTIR9D3

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

mIgG2b Fcγ Fragment Specific Rabbit monoclonal antibody,OTIR13C2

Applications WB 1:2000, ELISA 1:5000-1:20000
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (H+L chain) rabbit polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA : 1:50,000 - 1:300,000
Western Blot : 1:10,000 - 1:30,000
Immunohistochemistry: 1:500 - 1:2,500
Other Dilution: User Optimized
Reactivities Mouse
Conjugation HRP

Mouse IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications Suitable for ELISA, Immunofluorescence Microscopy.
Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
IF Microscopy: 1/1000-1/5000.
Reactivities Mouse
Conjugation FITC

Human IgG1 rabbit polyclonal antibody, Aff - Purified

Applications Western blot: 1/500-1/1000.
Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Reactivities Human, Mouse, Rat
Conjugation Unconjugated

Mouse IgE (Fc specific) rabbit polyclonal antibody, Azide Free

Applications Can be used as unlabelled secondary antibody for indirect detection of IgE in Mouse cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in Mouse serum or other body fluid. When applied in any Immunocytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO 488 Conjugated

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 488

Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody ATTO 488 Conjugated

Applications WB: >1:10,000
IF: >1:5,000
FLISA: >1:20,000
Reactivities Mouse
Conjugation ATTO 488

Mouse IgE (Fc specific) rabbit polyclonal antibody, Biotin

Applications Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgE at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology. In non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in mouse serum or other body fluid. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/3000.
Performance testing: Isotype specificity and quantitative specific recognition ability are further evaluated at the high level of sensitivity by direct singe and simultaneous double staining of different types of mouse cells, using counterstaining with reference conjugates with a different marker. The immunoconjugate is further tested in ELISA-type assays.
Reactivities Mouse
Conjugation Biotin