Primary Antibodies

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DYKDDDDK Epitope Tag chicken polyclonal antibody, Aff - Purified

Applications ELISA, IF, IHC, IP, WB
Conjugation Unconjugated
Immunogen Synthetic peptide containing the DYKDDDDK epitope conjugated to Keyhole Limpet Hemocyanin (KLH).
Production: The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were then affinity-purified over a peptide column and dialyzed against Phosphate-Buffered isotonic Saline. Sodium azide was added, the protein concentration was adjusted and the antibodies were filter-sterilized (0.2 μm).

GFP chicken polyclonal antibody, Aff - Purified

Applications IF, IHC, WB
Conjugation Unconjugated
Immunogen Purified recombinant Green Fluorescent Protein (GFP) emulsified in Freund's adjuvant.
After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks.
Purified antibody preparations were then mixed with Glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with Thimerosal, and then filter-sterilized.

GAD67 (GAD1) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen Synthetic peptide KLH conjugated corresponding to a region near the C-terminus of this gene product, and was 100% conserved between the Human (Q99259), Mouse (P48318) and Rat (NP_058703) gene products.
After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity purified using a peptide column.

Myelin Protein Zero (MPZ) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC, WB
Reactivities Human, Mouse
Conjugation Unconjugated
Immunogen Two antipeptide antibodies were generated in Chickens against sequences shared between the Mouse (NP_032649) and Human (NP_000521) gene products.
Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.2 mg/ml. Finally, equal volumes of both of these affinity purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Lepr (LepRb / OBRb) chicken polyclonal antibody

Applications IF, IHC, WB
Reactivities Rat
Conjugation Unconjugated

Nefl chicken polyclonal antibody

Applications IF, IHC, WB
Reactivities Feline, Mouse, Rat
Conjugation Unconjugated

Chicken Polyclonal ApoA1 Antibody

Applications IF, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen ApoA1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ApoA1. The immunogen is located within the first 50 amino acids of ApoA1.

Chicken Polyclonal erbB-2 Antibody

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen erbB-2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human erbB-2.

Chicken Polyclonal Transthyretin Antibody

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen Transthyretin antibody was raised against a 17 amino acid peptide near the center of human Transthyretin .

Chicken Polyclonal AP2M1 Antibody

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen AP2M1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human AP2M1.

Chicken Polyclonal CCDC69 Antibody

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen CCDC69 antibody was raised against a 16 amino acid peptide near the amino terminus of human CCDC69.

Chicken Polyclonal ENC-1 Antibody

Applications IF, IHC, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen ENC-1 antibody was raised against a 13 amino acid peptide near the center of human ENC-1.

Chicken Anti-Ubiquitin C-terminal Hydrolase 1 (UCHL1) ck Antibody

Applications IF, WB
Reactivities Human, Rat
Conjugation Unconjugated
Immunogen Recombinant full length human UCHL1 purified from E. coli

Chicken Polyclonal Anti-GAPDH Antibody

Applications IF, WB
Reactivities Human, Mouse, Rat
Conjugation Unconjugated
Immunogen GAPDH antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human GAPDH. The immunogen is located within amino acids 230 - 280 of GAPDH.

beta III Tubulin (TUBB3) chicken polyclonal antibody, Aff - Purified

Applications IF, IHC, WB
Reactivities Human, Rat
Conjugation Unconjugated
Immunogen Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Beta-Tubulin 3 gene product, but are shared between the Human (AAL28094) and Rat (AAM28438) protein sequences.
Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.