ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with an N-terminal mRFP (monomeric) tag.
mRFP is a monomeric red (orange) fluorescent protein generated from the wild-type RFP from sea anemone Entacmaea quadricolor. It possesses bright fluorescence with excitation/emission maxima at 555 and 584 nm, respectively. mRFP is about three times brighter than mCherry protein, which makes it the brightest monomeric red fluorescent protein available so far. mRFP is mainly intended for protein labeling and FRET applications. It can also be used for cell and organelle labeling and for tracking the promoter activity.
If the N-terminal tagging interferes with the protein’s function, you can choose the C-terminal mRFP-tagging vector, PS100041.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
The use of this Vectors has been cited in the following citations:
Efficient protein knockdown of HaloTag-fused proteins using hybrid molecules consisting of IAP antagonist and HaloTag ligand,Tomoshige, S;Hashimoto, Y;Ishikawa, M;,Bioorg. Med. Chem. May 2016,PubMed ID 27236416