ORFs cloned in this vector will be expressed in mammalian cells as a tagged protein with a C-terminal mYFP (monomeric) tag.
mYFP is a monomeric yellow fluorescent protein. mYFP possesses single excitation maximum at 508 nm, and emission maximum at 524 nm. mYFP is more pH stable than EYFP. mYFP is mainly intended for protein labeling in protein localization and interaction studies. It can also be used for cell and organelle labeling and for tracking the promoter activity, although TurboYFP is preferable for such applications because it mature faster and give brighter fluorescent signal.
If the C-terminal tagging interferes with the protein’s function, you can choose the N-terminal mYFP-tagging vector, PS100050.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
The use of this Vectors has been cited in the following citations:
The LIMP-2/SCARB2 binding motif on acid ß-glucosidase: basic and applied implications for Gaucher disease and associated neurodegenerative diseases,Liou, B;Haffey, WD;Greis, KD;Grabowski, GA;,J. Biol. Chem. Sep 2014,PubMed ID 25202012[PS100010]
Selective Recapitulation of Conserved and Non-Conserved Regions of Putative NOXA1 Activation Domain Confers Isoform-Specific Inhibition of Nox1 Oxidase, Attenuation of Endothelial Cell Nox and Migration,Ranayhossaini, DJ;Rodriguez, AI;Sahoo, S;Chen, BB;Mallampalli, RK;Kelley, EE;Csanyi, G;Gladwin, MT;Romero, G;Pagano, PJ;,J. Biol. Chem. Nov 2013,PubMed ID 24187133[pCMV6-AC-mYFP]