ATM Human shRNA Plasmid Kit (Locus ID 472)
ATM - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector
|Product Name||ATM Human shRNA Plasmid Kit (Locus ID 472)|
|Synonyms||AT1, ATA, ATC, ATD, ATE, ATDC, MGC74674, DKFZp781A0353|
|Kit Components||ATM - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 472). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.|
|RefSeq||BC007023, NM_000051, NM_138292, NM_138293, XM_005271561, XM_005271562, XM_006718843, XM_006718845, XM_011542840, XM_011542842, XM_011542843, XM_011542844, XM_011542845, XM_017017789, XM_017017790, XM_017017791, XM_017017792|
|Summary||The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010].|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
|The use of this RNAi has been cited in the following citations:|
ATM regulation of IL-8 links oxidative stress to cancer cell migration and invasion
,Chen, WT;Ebelt, ND;Stracker, TH;Xhemalce, B;Van Den Berg, CL;Miller, KM;,
Elife Jun 2015,PubMed ID 26030852