XRCC5 Human shRNA Plasmid Kit (Locus ID 7520)
XRCC5 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector
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|Product Name||XRCC5 Human shRNA Plasmid Kit (Locus ID 7520)|
|Synonyms||KARP-1; KARP1; KU80; Ku86; KUB2; NFIV|
|Kit Components||XRCC5 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 7520). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.|
|Summary||The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008].|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
|The use of this RNAi has been cited in the following citations:|
Dual Functions of the Homeoprotein DLX4 in Modulating Responsiveness of Tumor Cells to Topoisomerase II-Targeting Drugs
,Bon Q. Trinh, Song Yi Ko, Nicolas Barengo, Shiaw-Yih Lin, and Honami Naora,
Cancer Res., Jan 2013; 73: 1000 - 1010.