CD45 (PTPRC) (NM_002838) Human Recombinant Protein

CAT#: TP762398

Purified recombinant protein of Human protein tyrosine phosphatase, receptor type, C (PTPRC), transcript variant 1, Thr221-Gln396, with N-terminal His tag, expressed in E.coli, 50ug

Product Images

Purified recombinant protein CD45 was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding the region (Thr221-Gln396,) of CD45
• Tag: N-His
• Predicted MW: 20.0 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50 mM Tris-HCl, pH 8.0, 8 M urea
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_002829
• Locus ID: 5788
• UniProt ID: P08575
• Cytogenetics: 1q31.3-q32.1
• Refseq Size: 5026
• Refseq ORF: 3918
• Synonyms: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; T200
• Summary: The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Phosphatase, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Fc gamma R-mediated phagocytosis, Primary immunodeficiency, T cell receptor signaling pathway