BMI1 (NM_005180) Human Recombinant Protein

CAT#: TP760041

Recombinant protein of human BMI1 polycomb ring finger oncogene (BMI1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Product Images

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  BMI1 competitively inhibits the interaction between MDM2 and the AR-NTD. Purified AR-NTD and GST-MDM2 proteins were incubated with purified His-BMI1 protein (OriGene TP760041) at indicated concentrations at 4 C for 12 hours, followed by GST immunoprecipitation (IP). Both inputs and immunoprecipitated samples were probed with indicated antibodies in Western blot. Figure cited from Nat Commun, PMID: 29402932

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  CK2alpha phosphorylates BMI1 at serine 110. Purified BMI1 (OriGene TP760041) and CK2alpha reacted in a kinase assay buffer supplemented with nonradioactive ATP. The reaction sample was analyzed with mass spectrometry. The spectrum of the BMI1 phosphopeptide is presented with the extracted ion chromatogram for the peptide with phosphate shown in the inset (indicated by #). Figure cited from Mol Cancer, PMID: 28270146

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding human full-length BMI1
• Tag: N-His
• Predicted MW: 36.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 100 mM arginine, 10% glycerol
• Bioactivity: In vitro kinase assay substrate (PMID: 28270146)
  Binding assay (competitor) (PMID: 29402932)
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_005171
• Locus ID: 648
• UniProt ID: P35226
• Cytogenetics: 10p12.2
• Refseq Size: 3435
• Refseq ORF: 978
• Synonyms: flvi-2/bmi-1; FLVI2/BMI1; PCGF4; RNF51
• Summary: This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene. [provided by RefSeq, Sep 2015]
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors