SEPSECS (NM_016955) Human Recombinant Protein

CAT#: TP750207

Purified recombinant protein of human Sep (O-phosphoserine) tRNA:Sec (selenocysteine) tRNA synthase, full length, with N-terminal His tag, expressed in E.coli, 50ug

Product Images

Purified recombinant protein SEPSECS was analyzed by SDS-PAGE gel and Coomossie Blue Staining.

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MHHHHHHMDS NNFLGNCGVG EREGRVASAL VARRHYRFIH GIGRSGDISA VQPKAAGSSL
  LNKITNSLVL DIIKLAGVHT VANCFVVPMA TGMSLTLCFL TLRHKRPKAK YIIWPRIDQK
  SCFKSMITAG FEPVVIENVL EGDELRTDLK AVEAKVQELG PDCILCIHST TSCFAPRVPD
  RLEELAVICA NYDIPHIVNN AYGVQSSKCM HLIQQGARVG RIDAFVQSLD KNFMVPVGGA
  IIAGFNDSFI QEISKMYPGR ASASPSLDVL ITLLSLGSNG YKKLLKERKE MFSYLSNQIK
  KLSEAYNERL LHTPHNPISL AMTLKTLDEH RDKAVTQLGS MLFTRQVSGA RVVPLGSMQT
  VSGYTFRGFM SHTNNYPCAY LNAASAIGMK MQDVDLFIKR LDRCLKAVRK ERSKESDDNY
  DKTEDVDIEE MALKLDNVLL DTYQDASS
• Tag: N-His
• Predicted MW: 49.7 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: PBS, pH 7.4, 1% sarkosyl, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_058651
• Locus ID: 51091
• UniProt ID: Q9HD40
• Cytogenetics: 4p15.2
• Synonyms: LP; PCH2D; SLA; SLA/LP
• Summary: The amino acid selenocysteine is the only amino acid that does not have its own tRNA synthetase. Instead, this amino acid is synthesized on its cognate tRNA in a three step process. The protein encoded by this gene catalyzes the third step in the process, the conversion of O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec).[provided by RefSeq, Mar 2011]
• Protein Pathways: Aminoacyl-tRNA biosynthesis