CD28 Human Recombinant Protein

CAT#: TP723937

Human CD28 Protein, mFc-His Tag

Product Images

Figure 1. Human CD28 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

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  Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD28, mFc-His tagged protein (TP723937) can bind Human B7-1, hFc tagged protein (TP724008) in a linear range of 125-4000 ng/ml.

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  Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD28, mFc-His tagged protein (TP723937) can bind Human B7-2, hFc tagged protein (TP724009) in a linear range of 62.5-4000 ng/ml.

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  Figure 4. HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28, mFc-His tagged protein (TP723937) 1µg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  CD28（Asn19-Pro152）+mFc（Pro99-Lys330）+6×His
• Tag: C-Mouse Fc and 6×His
• Predicted MW: 68-72 kDa
• Purity: > 95%
• Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization
• Reconstitution: Reconstitute with deionized water
• Preparation: Affinity purification
• Applications: ELISA, Flow Cyt
• Storage: Store the lyophilized protein at -20°C.
  After reconstitution, store the protein at -80°C for 12 months.
  Avoid repeated freezing and thawing.
• Stability: 12 months from date of despatch

Reference Data

• Locus ID: 940
• UniProt ID: P10747
• Summary: The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.