CD47 Human Recombinant Protein

CAT#: TP723931

Human CD47 Protein, mFc-His Tag

Product Images

Figure 1. Human CD47, mFc-His Tag on SDS-PAGE under reducing condition.

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  Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD47, mFc-His tagged protein (TP723931) can bind its native ligand Human SIRPα, hFc-His tagged protein (TP723932) in a linear range of 3.3-26.37 ng/ml.

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  Figure 3. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD47, mFc-His tagged protein (TP723931) can bind Anti-CD47 (Neutralizing antibody clone Hu5F9-G4) (TA355193) in a linear range of 0.061-1.606 ng/ml.

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  CD47（Gln19-Gln141）+CD47（Val198-Ala207）+CD47（Ser257-Gly268）+mFc（Pro99-Lys330）+6×His tag
• Tag: C-Mouse Fc and 6×His
• Predicted MW: 60 kDa
• Purity: > 95%
• Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization
• Reconstitution: Reconstitute with deionized water
• Preparation: Affinity purification
• Applications: ELISA
• Storage: Store the lyophilized protein at -20°C.
  After reconstitution, store the protein at -80°C for 12 months.
  Avoid repeated freezing and thawing.
• Stability: 12 months from date of despatch

Reference Data

• Locus ID: 961
• UniProt ID: Q08722
• Summary: Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.