TSLP (NM_033035) Human Recombinant Protein

CAT#: TP723796

Purified recombinant protein of Human thymic stromal lymphopoietin (TSLP), transcript variant 1

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human TSLP,the rehion of Met- Tyr29-Gln159, from gene accession# NM_033035.4
• Tag: Tag Free
• Predicted MW: 15 kDa
• Concentration: lot specific
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 20 mM NaHPO4, pH 7.2, 0.15 M NaCl
• Bioactivity: The ED50 is 1 - 5 ng/ml, corresponding to a specific activity of 0.2 - 1 x 10^6 units/mg, determined by the dose dependent stimulation of mouse BaF3-human IL-7a and human TSLPR cotransfectant cell proliferation.
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_149024
• Locus ID: 85480
• UniProt ID: Q969D9
• Cytogenetics: 5q22.1
• Refseq Size: 2652
• Refseq ORF: 477
• Summary: This gene encodes a hemopoietic cytokine proposed to signal through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. It mainly impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes and enhances the maturation of CD11c(+) dendritic cells. The protein promotes T helper type 2 (TH2) cell responses that are associated with immunity in various inflammatory diseases, including asthma, allergic inflammation and chronic obstructive pulmonary disease. The protein is therefore considered a potential therapeutic target for the treatment of such diseases. In addition, the shorter (predominant) isoform is an antimicrobial protein, displaying antibacterial and antifungal activity against B. cereus, E. coli, E. faecalis, S. mitis, S. epidermidis, and C. albicans. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2020]
• Protein Families: Druggable Genome
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway