CD274 Human Recombinant Protein

CAT#: TP721259M

Human PD-L1 Protein (C-Fc)

Product Images

Functional ELISA assay: Streptavidin is immobilized at 2ug/mL, 100 µL/well. Followed by biotinylated Human PD1 (C-His-Avi) at 0.5ug/mL and a series dilution of recombinant Human PD-L1 (C-Fc). HRP Anti-hIgG1 secondary antibody (1:5000) is used as detection reagent. The results showed 50% of the optimal binding response is approximately 6 ng/mL.

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  Human PD-L1 protein (C-Fc) on SDS-PAGE under reducing condition P(+) and non-reducing condition P(-) . The purity of this protein appears to be greater than 95% based on Coomassie-blue staining.

Specifications

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Phe19-Thr239
• Tag: C-Fc
• Predicted MW: The protein has a predicted molecular weight of 53kDa. The protein migrates at approximately 70kDa on SDS-PAGE with DTT-reduced conditions.
• Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
• Purity: >90%
• Presentation: Purified
• Buffer: 1xPBS buffer, pH7.4
• Bioactivity: Positive
  
  The definition of the active protein (purified and biotinylated) is defined as the protein that can bind to its biological receptor/ligand. For conjugated protein, it is defined with its function to bind to the ScFv of the active CAR-transfected cells in flow cytometry test.
• Preparation: Affinity Protein A
• Applications: ELISA
• Storage: An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles.
• Stability: 6 Months

Reference Data

• RefSeq: NP_054862.1
• Locus ID: 29126
• UniProt ID: Q9NZQ7