FCGR1A Human Recombinant Protein

CAT#: TP721250M

Human CD64 Protein (C-His-Avi)

Product Images

Human IgG1 isotype protein is coated at 1 ug/mL (100uL/well). Human CD64 protein can be bind Human IgG1 protein in the dose dependent manner. The EC50 is about 3-10 ng/mL.

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  Human CD64 protein (C-His-Avi) on SDS-PAGE under reducing condition P(+) and non-reducing condition P(-) . The purity of this protein appears to be greater than 95% based on Coomassie-blue staining.

Specifications

Product Data

• Species: Human
• Expression Host: CHO
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Gln16-Thr287
• Tag: C-His-Avi
• Predicted MW: The protein has the predicted molecular weight of 34.2kDa and migrates at approximately 50-60 kDa on SDS-PAGE with DTT-reduced condition before PE conjugatoin.
• Concentration: 25µg size is bottled at 0.2mg/mL concentration. 100 µg size is bottled at lot specific concentration.
• Purity: >90%
• Presentation: Purified
• Buffer: 1xPBS buffer, pH7.4
• Bioactivity: Positive
  
  The definition of the active protein (purified and biotinylated) is defined as the protein that can bind to its biological receptor/ligand. For conjugated protein, it is defined with its function to bind to the ScFv of the active CAR-transfected cells in flow cytometry test.
• Preparation: Affinity Ni-NTA
• Applications: ELISA
• Storage: An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles.
• Stability: 6 Months

Reference Data

• RefSeq: NP_000557.1
• Locus ID: 2209
• UniProt ID: P12314